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KMID : 0043319900130010028
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Archives of Pharmacal Research
1990 Volume.13 No. 1 p.28 ~ p.32
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Precepitation and Purification of Amylase Enzyme Produced by Streptomyces aureofaciens 77
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Ibrahim AN
Ahmed FH/Ibrahim MMK/Arafa MAI
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Abstract
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Precipitation and purification of amylase secreted by Streptomyces aureofaciens 77 in liquid inorganic salts-starch medium under the optimum conditions were carried out. Ammonium sulphate fractionation was used to precipitate amylase in cell free culture filtrate. (NH4)2SO4 at a concentration of 50-70% saturation gave the highest enzyme yield. The obtained precipitates were redissolved in phosphate buffer (pH 7.0) and subjected to dialysis. The dialyzed enzyme preparation was applied to DEAE-cellulose column chromatography which resulted in an increase of purification up to 59.48 fold. A further step of purification was done by applying the obtained purified sample to Sephadex-G200 column chromatography which resulted in an increase of purification up to 73.92 fold. The results clearly indicated that the isolated amylase from S. aureofaciens 77 was only on type.
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